Laser Assisted Hatching

An embryo must hatch out of its outer membrane (zona pellucid) before implanting in the uterine wall (endometrium). Sometimes, the zona is abnormally thick. Laser assisted hatching is a technique that allows a small gap in the zona pellucida to be made. best ivf center in pakistan This will aid the embryo in breaking out of this membrane and facilitates implantation. It is a technique that is performed before embryo transfer and when doing trophectoderm biopsies. Assisted hatching is specifically recommended for patients who are over 37 years of age, have diminished ovarian reserve as determined by a day 3 FSH level, or have lower antral follicle counts. Patients who are poor responders to gonadotropin stimulation, or have had previous failed implantation may also benefit from this procedure. Studies have shown that assisted hatching improves IVF success rates in both fresh embryo transfers and frozen embryo transfers.

Cryopreservation

Embryo cryopreservation (freezing) may be available to patients that have an excess number of normally fertilized embryos or high quality blastocysts that remain following embryo transfer. Embryos may be frozen at the zygote stage one day after egg retrieval, or on day 5 or 6 at the blastocyst stage.

Microsurgical Epididymal Sperm Aspiration (MESA) or Testicular Sperm Extraction (TESE)

Some patients’ semen samples contain no spermatozoa due to a congenital obstruction of the sperm ducts, vasectomy, failed vasectomy reversal, or primary testicular failure. In these conditions, a urologist can obtain sperm surgically from the epididymis (MESA) or from the testis (TESE). This sperm can be frozen and used for fertilization by ICSI.

Embryo Biopsy

Preimplantation Genetic Testing (PGT)

PGT is a procedure that is performed in conjunction with IVF. It is designed to help detect genetic abnormalities/inherited genetic diseases in embryos before implantation, thereby minimizing the transfer of affected embryos.

Preimplantation Genetic Testing – Aneuploidy (PGT-A)

Normal human cells contain 46 chromosomes located in the nucleus of the cell. Chromosomes carry the genetic information in the form of DNA. Every human being receives 23 chromosomes from each parent. If an error occurs leading to the egg or sperm having a missing or extra chromosome, the embryo created will also have a missing or extra chromosome. This condition is called aneuploidy. Most of the aneuploidies will not result in implantation of the embryo, but certain aneuploidies, such as trisomy 21, can implant and lead to Down’s syndrome. Some other common aneuploidies include trisomy 13, trisomy 18, and Klinefelter’s syndrome (XXY).

Indications for PGT

PGT is indicated for patients who have a history of recurrent miscarriages, advanced maternal age (≥38 years), repeated IVF failures in spite of high grade embryos, unexplained infertility, severe male factor infertility, or inherited genetic disorders (e.g., cystic fibrosis, Tay Sachs disease, Myotonic dystrophy, etc.). Currently, there are more than 1000 types of single gene mutations that can be diagnosed.

Procedure

In order to perform genetic testing on an embryo, several cells from the trophectoderm layer are extracted on fifth, sixth or seventh day of development.test tube baby in lahore This procedure is called an embryo biopsy. The extracted cells are sent out for analysis to Cooper Genomics and/or Ingenomix. Subsequently, the biopsied embryo is vitrified. After the results are received, patients will have the normal embryo transferred back.

Benefits of PGT

Patients who are carriers of single gene mutations can avoid transmitting those disorders to their offspring by testing the embryos and choosing not to transfer those which are affected or carriers.

Patients who have had several miscarriages in the past can benefit from PGT-A for aneuploidy screening by avoiding the transfer of embryos that are aneuploid and will eventually fail to implant. The likelihood of having a trisomic pregnancy increases with advanced maternal age (>38 years). PGT-A allows selection of normal embryos at the pre-implantation stage and reduces the chance of detecting abnormal fetal development during an aminocentesis in the second trimester. Patients who failed several IVF attempts and are known to generate good cohorts of embryos might have a high rate of aneuploidy and can benefit by PGT-A. Another group of patients who can benefit from PGT are those who carry translocations, which are detected by karyotyping.

Risks

There are certain risks associated with any micromanipulation procedure, but these are minimal. Trophectoderm biopsy does require embryos to develop to the blastocyst stage before the biopsy is performed. Not all couples will have embryos that develop to this stage. Occasionally, results are not able to be determined.Additional IVF Procedures
Hatching Blast
Laser Assisted Hatching
An embryo must hatch out of its outer membrane (zona pellucid) before implanting in the uterine wall (endometrium). Sometimes, the zona is abnormally thick. Laser assisted hatching is a technique that allows a small gap in the zona pellucida to be made. This will aid the embryo in breaking out of this membrane and facilitates implantation. It is a technique that is performed before embryo transfer and when doing trophectoderm biopsies. Assisted hatching is specifically recommended for patients who are over 37 years of age, have diminished ovarian reserve as determined by a day 3 FSH level, or have lower antral follicle counts. Patients who are poor responders to gonadotropin stimulation, or have had previous failed implantation may also benefit from this procedure. Studies have shown that assisted hatching improves IVF success rates in both fresh embryo transfers and frozen embryo transfers.
Cryopreservation
Embryo cryopreservation (freezing) may be available to patients that have an excess number of normally fertilized embryos or high quality blastocysts that remain following embryo transfer. Embryos may be frozen at the zygote stage one day after egg retrieval, or on day 5 or 6 at the blastocyst stage. 
Microsurgical Epididymal Sperm Aspiration (MESA) or Testicular Sperm Extraction (TESE)
Some patients’ semen samples contain no spermatozoa due to a congenital obstruction of the sperm ducts, vasectomy, failed vasectomy reversal, or primary testicular failure. In these conditions, a urologist can obtain sperm surgically from the epididymis (MESA) or from the testis (TESE). This sperm can be frozen and used for fertilization by ICSI.
Embryo Biopsy
Preimplantation Genetic Testing (PGT)
PGT is a procedure that is performed in conjunction with IVF. It is designed to help detect genetic abnormalities/inherited genetic diseases in embryos before implantation, thereby minimizing the transfer of affected embryos.
Preimplantation Genetic Testing – Aneuploidy (PGT-A)
Normal human cells contain 46 chromosomes located in the nucleus of the cell. Chromosomes carry the genetic information in the form of DNA. Every human being receives 23 chromosomes from each parent. If an error occurs leading to the egg or sperm having a missing or extra chromosome, the embryo created will also have a missing or extra chromosome. This condition is called aneuploidy. Most of the aneuploidies will not result in implantation of the embryo, but certain aneuploidies, such as trisomy 21, can implant and lead to Down’s syndrome. Some other common aneuploidies include trisomy 13, trisomy 18, and Klinefelter’s syndrome (XXY).
Indications for PGT
PGT is indicated for  patients who have a history of recurrent miscarriages, advanced maternal age (≥38 years), repeated IVF failures in spite of high grade embryos, unexplained infertility, severe male factor infertility, or inherited genetic disorders (e.g., cystic fibrosis, Tay Sachs disease, Myotonic dystrophy, etc.). Currently, there are more than 1000 types of single gene mutations that can be diagnosed.
Procedure
In order to perform genetic testing on an embryo, several cells from the trophectoderm layer are extracted on fifth, sixth or seventh day of development. This procedure is called an embryo biopsy. The extracted cells are sent out for analysis to Cooper Genomics and/or Ingenomix. Subsequently, the biopsied embryo is vitrified. After the results are received, patients will have the normal embryo transferred back.
Benefits of PGT
Patients who are carriers of single gene mutations can avoid transmitting those disorders to their offspring by testing the embryos and choosing not to transfer those which are affected or carriers.
Patients who have had several miscarriages in the past can benefit from PGT-A for aneuploidy screening by avoiding the transfer of embryos that are aneuploid and will eventually fail to implant. The likelihood of having a trisomic pregnancy increases with advanced maternal age (>38 years). PGT-A allows selection of normal embryos at the pre-implantation stage and reduces the chance of detecting abnormal fetal development during an aminocentesis in the second trimester. Patients who failed several IVF attempts and are known to generate good cohorts of embryos might have a high rate of aneuploidy and can benefit by PGT-A. Another group of patients who can benefit from PGT are those who carry translocations, which are detected by karyotyping.

Additional IVF Procedures

Hatching Blast

Laser Assisted Hatching

An embryo must hatch out of its outer membrane (zona pellucid) before implanting in the uterine wall (endometrium). Sometimes, the zona is abnormally thick. Laser assisted hatching is a technique that allows a small gap in the zona pellucida to be made. male infertility clinic in lahore This will aid the embryo in breaking out of this membrane and facilitates implantation. It is a technique that is performed before embryo transfer and when doing trophectoderm biopsies. Assisted hatching is specifically recommended for patients who are over 37 years of age, have diminished ovarian reserve as determined by a day 3 FSH level, or have lower antral follicle counts. Patients who are poor responders to gonadotropin stimulation, or have had previous failed implantation may also benefit from this procedure. Studies have shown that assisted hatching improves IVF success rates in both fresh embryo transfers and frozen embryo transfers.

Cryopreservation

Embryo cryopreservation (freezing) may be available to patients that have an excess number of normally fertilized embryos or high quality blastocysts that remain following embryo transfer. Embryos may be frozen at the zygote stage one day after egg retrieval, or on day 5 or 6 at the blastocyst stage.

Microsurgical Epididymal Sperm Aspiration (MESA) or Testicular Sperm Extraction (TESE)

Some patients’ semen samples contain no spermatozoa due to a congenital obstruction of the sperm ducts, vasectomy, failed vasectomy reversal, or primary testicular failure. In these conditions, a urologist can obtain sperm surgically from the epididymis (MESA) or from the testis (TESE). This sperm can be frozen and used for fertilization by ICSI.

Embryo Biopsy

Preimplantation Genetic Testing (PGT)

PGT is a procedure that is performed in conjunction with IVF. It is designed to help detect genetic abnormalities/inherited genetic diseases in embryos before implantation, thereby minimizing the transfer of affected embryos.

Preimplantation Genetic Testing – Aneuploidy (PGT-A)

Normal human cells contain 46 chromosomes located in the nucleus of the cell. Chromosomes carry the genetic information in the form of DNA. Every human being receives 23 chromosomes from each parent. If an error occurs leading to the egg or sperm having a missing or extra chromosome, the embryo created will also have a missing or extra chromosome. This condition is called aneuploidy. Most of the aneuploidies will not result in implantation of the embryo, but certain aneuploidies, such as trisomy 21, can implant and lead to Down’s syndrome. Some other common aneuploidies include trisomy 13, trisomy 18, and Klinefelter’s syndrome (XXY).

Indications for PGT

PGT is indicated for patients who have a history of recurrent miscarriages, advanced maternal age (≥38 years), repeated IVF failures in spite of high grade embryos, unexplained infertility, severe male factor infertility, or inherited genetic disorders (e.g., cystic fibrosis, Tay Sachs disease, Myotonic dystrophy, etc.). Currently, there are more than 1000 types of single gene mutations that can be diagnosed.

Procedure

In order to perform genetic testing on an embryo, several cells from the trophectoderm layer are extracted on fifth, sixth or seventh day of development. This procedure is called an embryo biopsy. The extracted cells are sent out for analysis to Cooper Genomics and/or Ingenomix. Subsequently, the biopsied embryo is vitrified. After the results are received, patients will have the normal embryo transferred back.

Benefits of PGT

Patients who are carriers of single gene mutations can avoid transmitting those disorders to their offspring by testing the embryos and choosing not to transfer those which are affected or carriers.

Patients who have had several miscarriages in the past can benefit from PGT-A for aneuploidy screening by avoiding the transfer of embryos that are aneuploid and will eventually fail to implant. The likelihood of having a trisomic pregnancy increases with advanced maternal age (>38 years). PGT-A allows selection of normal embryos at the pre-implantation stage and reduces the chance of detecting abnormal fetal development during an aminocentesis in the second trimester. infertility doctors in Pakistan Patients who failed several IVF attempts and are known to generate good cohorts of embryos might have a high rate of aneuploidy and can benefit by PGT-A. Another group of patients who can benefit from PGT are those who carry translocations, which are detected by karyotyping.

Risks

There are certain risks associated with any micromanipulation procedure, but these are minimal. Trophectoderm biopsy does require embryos to develop to the blastocyst stage before the biopsy is performed. Not all couples will have embryos that develop to this stage. Occasionally, results are not able to be determined.

There are certain risks associated with any micromanipulation procedure, but these are minimal. Trophectoderm biopsy does require embryos to develop to the blastocyst stage before the biopsy is performed. Not all couples will have embryos that develop to this stage. Occasionally, results are not able to be determined.